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The result is phrase of a recombinant protein with a 6xHis or poly-His-tag fused to its N- or C-terminus

The result is phrase of a recombinant protein with a 6xHis or poly-His-tag fused to its N- or C-terminus The DNA sequence indicating a string of six to nine histidine residues is frequently included in vectors for creation of recombinant healthy proteins. Expressed His-tagged healthy proteins tends to be purified and recognized conveniently due to the fact string of histidine deposits binds to several different immobilized material ions, like nickel,