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An additional applying of our UTF1 reporter cassette ( 31), i employed target vector pattP4X-PGKssPuro-UTF1-EGFP

An additional applying of our UTF1 reporter cassette ( 31), i employed target vector pattP4X-PGKssPuro-UTF1-EGFP Right here, brand new UTF1 supporter/enhancer factors handle EGFP phrase, since puromycin resistance cassette was constitutively indicated just after directed genomic combination (Figure 4A). Co-transfection of your target vector pattP4X-PGKssPuro-UTF1-EGFP and you may Int-C3NLS term vector followed by options with puromycin led to 120 colonies. Original PCR examination having fun with ORF1 submit primer followed

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